Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 100G
Sigma Aldrich Fine Chemicals Biosciences MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g
MES hydrate BioUltra | 1266615-59-1 | MFCD00149409 | 250g
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 25 g
MES monohydrate | Purity: ≥99.0% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 25 g
Sigma Aldrich Fine Chemicals Biosciences MES hydrate | 1266615-59-1 | MFCD00149409 | 50 g
MES hydrate | Purity: ≥99.5% | Mol Wt: 195.24 | 1266615-59-1 | MFCD00149409 | 50 g
Sigma Aldrich Fine Chemicals Biosciences MES hydrate >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
MES hydrate >=99.5% (titration) | Purity: >=99.5% (titration) | 1266615-59-1 | MFCD00149409 | 250G
United States Biological Corporation DULBECCO MEM DMEM PWDR D980028
NC3314668 DULBECCO MEM DMEM PWDR D980028
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Biotang Inc TRIS-HEPES-Native Running Buffer (10X), 500 ml
TRIS-HEPES-Native Running Buffer (10X), 500 ml.
Biotang Inc TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml
TRIS-Buffered Saline (TBS, 10X) For Western Blot Washing; (NaCl 1.5M Tris 0.5M), 1000 ml.
Biotang Inc Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml
Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml.