Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Promocell GmbH PromoCell Trypsin / EDTA 30 ml, 0.04 % Trypsin / 0.03 % EDTA
Trypsin is still the most widely employed enzyme used for cell detachment. PromoCell´s Trypsin Solutions exhibit standardized enzyme activity and contain trypsin from extra-pure lots.Trypsin / EDTA Solution having a ratio of 0.04% / 0.03%.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The most effective strategy to enhance the yield of intact proteins is through the addition of inhibitors targeting these known endogenous enzymes The Protease Inhibitor Cocktail is intended for the purification of His-tagged proteins to improve protein stability This cocktail functions by inhibiting proteases that degrade either phosphorylated or non-phosphorylated protein substrates This Protease Inhibitor Cocktail contains individual components including AEBSF Bestatin E-64 Phosphoramidon and Pepstatin A offering broad-spectrum specific inhibition against cysteine serine aspartic thermolysin-like proteases and aminopeptidases It has been optimized and validated specifically for use with His-tagged proteins The cocktail is dissolved in DMSO and supplied as a ready-to-use solution
Boston Bioproducts Inc MES Buffer (1 M, pH 6.0) - 500ml
MES Buffer [2- (N-morpholino) ethanesulfonic acid] is a zwitterionic Good's buffer used in biological and biochemical research. It maintains a stable pH within the range of 5.5 to 7.0 and is highly soluble in water. MES is commonly used as a running buffer for bis-tris gels, chromatography, and in protein purification. It is valuable for enzymatic reactions, and acts as a non-coordinating buffer in metal ion chemistry. It has minimal UV absorbance and is stable in various applications.
Promocell GmbH PromoCell Dulbeccos PBS pH 7,2-7,8 500 ml, Standard, with Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Asuragen Inc TSMIII Storage Buffer, 50 mL, 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III, pH 7.0, -15 to -30°C
Asuragen TSMIII Storage Buffer is a specialized buffered solution designed to maintain stability for protein-coated nucleic acids. This reagent is ideal for the dilution of Asuragen's Armored products, ensuring the integrity of these sensitive biological materials through multiple freeze-thaw cycles. The stability provided by this storage buffer allows for flexible and scalable testing processes in various molecular assay applications.
- Volume: 50 mL
- Composition: 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III
- pH: 7.0
- Primary application: Dilution of Asuragen's Armored products
- Key benefit: Provides stability to protein-coated nucleic acids
- Storage stability: Withstands multiple freeze-thaw cycles
- Recommended storage temperature: -15 to -30 °C (secondary)
- Testing process benefit: Enables flexible and scalable testing processes
HIMEDIA Dulbeccos Phosphate Buffered
Dulbecco s Phosphate Buffered Saline 1X w/o Phenol red Calcium and Magnesium
Alkali Scientific QuickSilver™ Buffer Powder, Tris-MOPS-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra suitable for500G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.
Teknova 1M HEPES, pH 7.0. 1000mL, Sterile
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Teknova 10X DPBS with 0.5% Tween 20, pH 7.4. 1000mL, Non-Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.