Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc HEPES, Free Acid 250 g
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, for Western Blot Washing) - 1L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 10X concentration, and is useful for washing in Western Blotting.
Alkali Scientific CellPro™ PBS Buffer, 1X, pH 7.4, without Calcium or Magnesium, Sterile Filtered, 1000mL, 10/Case
CellPro™ PBS Buffer, 1X, pH 7.4, 1000mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (10X) - 1L
Dulbecco s Phosphate Buffered Saline DPBS (10X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
Phosphate Buffered Saline-Tween PBST (20X with 1% Tween-20 pH 7 4) - 4x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 8.5) - 500ml
HEPES Buffer (1 M pH 8 5) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bovine Serum Albumin, BSA (30% in DPBS) - 4x50ml
Bovine Serum Albumin BSA (30% in DPBS) - 4x50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.4) - 500ml
Sodium Phosphate Buffer (0 2 M pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bis-Tris Transfer Buffer (20X) - 1L
Bis-Tris Transfer Buffer (20X) - 1L (For Research Use and Further Manufacturing Only)
Aniara Diagnostica LLC TBS TBLT PH 7.6 500ML 100 EA
Medicago Tris Buffered Saline 1x (TBS) pH 7.6 supplied as preweighed tablets yields 500 mL 0.050 Tris-HCl, 0.15 M KCl with pH 7.6 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
Aniara Diagnostica LLC Phosphate Buffered Saline, pH 7.4, 100 tablets
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 500 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.